A micro method for detecting indol formation.
نویسنده
چکیده
The acid in the commonly used indol reagent described by Kovacs (1928, 1932) and Diagnostic Procedures and Reagents (1950) changes the reaction of the medium in which the indol production is tested and prevents the further observation of biochemical processes, killing the bacteria themselves. It was found that, by heating 0.1 ml. or less of culture (broth or peptone water), indol production by enteric bacteria could be easily detected. Four capillary drops of the fluid medium to be tested are put into a test tube of about 12 by 75 mm. and 4 drops of the indol reagent are added from a dropping bottle. The mixture is shaken lightly and if it does not turn red immediately it should be heated gently for a moment, without actual boiling, in the flame of a Bunsen burner. Heating doubles the sensitivity of the reaction seen at room temperature. For routine work the culture fluid which is to be tested is collected with a spiral-ended wire. This is
منابع مشابه
A method for detecting bubbles in two-phase gas-liquid flow
Detecting bubble in two-phase flow has been a basic issue in two-phase flow systems. A new method for measuring the frequency of bubble formation is presented in this paper. For this purpose, an electronic device was designed and constructed which works based on a change in intensity of laser beam. For this purpose, continues light beam is embedded just above the needle, which is received by a ...
متن کاملComparison of Micro Neutralization Test with Hemadsorption-Inhibition Test, and Immunoflrorescence Technique for Detecting Mumps Antibodies
متن کامل
A Simple Method for Detecting and Estimating Indican in the Urine by Means of the Cotton-Wool Plug Test
Indican is derived from indol, and unlike the other ethereal sulphates which are derived in part from metabolism, originates practically wholly from putrefactive processes. It, alone, therefore, and not the total ethereal sulphates, has been taken as an index of such putrefaction (1). The determination of indican depends upon its decomposition and oxidation of the liberated indoxyl into indigo-...
متن کاملCatalyst and Solvent-Free Microwave Assisted Expeditious Synthesis of 3-Indolyl-3-hydroxy Oxindoles and Unsymmetrical 3,3-Di(indolyl)indolin-2-ones
A simple and efficient method for the synthesis of 3-indolyl-3-hydroxy oxindoles and unsymmetrical 3,3-di(indolyl)indolin-2-ones using microwave irradiation without catalyst and solvent is described. A series of 3-indolyl-3-hydroxy oxindoles and unsymmetrical 3,3-di(indolyl)indolin-2ones have been synthesized in very short reaction times of 5 and 10 minutes and in yields ranging from 31% to 98%...
متن کاملInvestigation of physical conditions in the formation of micro and Nano iron/alginate capsules in the coacervation technique
Microencapsulation is a process in which solid, liquid or gas components are covered with a septum.The present study has focused on the effect of stirring to produce ferric saccharide capsules withalginate coating applying the coacervation method so that we can obtain the best capsules forfortification of hydrated and dehydrated food products. At first, three methods, including stirrer,ultra-so...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of clinical pathology
دوره 12 1 شماره
صفحات -
تاریخ انتشار 1959